What is one negative impact of pesticide overuse on non-target organisms?

The choice highlighting the development of resistance is significant because overusing pesticides creates selective pressure on pest populations. When pesticides are applied frequently, only those individuals that have or develop resistance genes are likely to survive and reproduce. Over time, this leads to a population of pests that are increasingly difficult to control, as they have evolved mechanisms to withstand the chemical effects of the pesticides. This resistance not only hampers future pest management efforts but also can lead to higher pesticide usage, resulting in an escalating cycle of pest resistance and increased environmental impact.

The other options do not align with the negative consequences of pesticide overuse. While increased biodiversity and improved ecological balance would typically be seen as positive outcomes, they are not associated with the direct effects of overusing pesticides. Moreover, enhanced pest control methods would ideally represent a benefit rather than a negative impact. Therefore, the development of resistance stands out as a crucial concern linked to the overuse of pesticides, affecting the effectiveness of pest control strategies and the health of the ecosystem.